hypothalamic expression of melanocortin-4 receptor and agouti-related peptide mrnas during the estrous cycle of rats

نویسندگان

mohammad reza zandi department of animal sciences, school of agriculture, shiraz university, shiraz, iran.سازمان اصلی تایید شده: دانشگاه شیراز (shiraz university)

mohammad reza jafarzadeh shirazi department of animal sciences, school of agriculture, shiraz university, shiraz, iran.سازمان اصلی تایید شده: دانشگاه شیراز (shiraz university)

amin tamadon transgenic technology research center, shiraz university of medical sciences, shiraz, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (shiraz university of medical sciences)

amir akhlaghi department of animal sciences, school of agriculture, shiraz university, shiraz, iran.سازمان اصلی تایید شده: دانشگاه شیراز (shiraz university)

چکیده

melanocortin- 4 receptor (mc4r) and agouti- related peptide (agrp) are involved in energy homeostasis in rats. according to mc4r and agrp effects on luteinizing hormone (lh) secretion, they may influence the estrous cycle of rats. therefore, the aim of this study was to investigate the expression of mc4r and agrp mrnas at different stages of estrous cycle in the rat’s hypothalamus. the estrous cycle stages (proestrus, estrus, metestrus and diestrus) were determined in 20 adult female rats using vaginal smears. the rats were divided into four equal groups (n=5). four ovariectomized rats were selected as controls two weeks after surgery. using real- time pcr, relative expressions (compared to controls) of mc4r and agrp mrnas in the hypothalamus of rats were compared in four different groups of estrous cycle. the relative expression of mc4r mrna in the hypothalamus of female rats during proestrus stage was higher than those in other stages (p=0.001). despite a lower mean of relative expression of agrp mrna at proestrus stage, the relative expression of agrp mrna of the four stages of estrous cycle did not differ (p>0.05). in conclusion, changes in the relative expression of mc4r and agrp mrnas in four stages of rat estrous cycle indicated a stimulatory role of mc4r in the proestrus and preovulatory stages and an inhibitory role of agrp in gonadotropin releasing hormone (gnrh) and lh secretions.

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منابع مشابه

Hypothalamic Expression of Melanocortin-4 Receptor and Agouti-related Peptide mRNAs During the Estrous Cycle of Rats

Melanocortin- 4 receptor (MC4R) and agouti- related peptide (AgRP) are involved in energy homeostasis in rats. According to MC4R and AgRP effects on luteinizing hormone (LH) secretion, they may influence the estrous cycle of rats. Therefore, the aim of this study was to investigate the expression of MC4R and AgRP mRNAs at different stages of estrous cycle in the rat’s hypothalamus. The estrous ...

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Hypothalamic Expression of Melanocortin-4 Receptor and Agouti-related Peptide mRNAs During the Estrous Cycle of Rats

Melanocortin- 4 receptor (MC4R) and agouti- related peptide (AgRP) are involved in energy homeostasis in rats. According to MC4R and AgRP effects on luteinizing hormone (LH) secretion, they may influence the estrous cycle of rats. Therefore, the aim of this study was to investigate the expression of MC4R and AgRP mRNAs at different stages of estrous cycle in the rat's hypothalamus. The estrous ...

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Expression of melanocortin-4 receptor and agouti-related peptide mRNAs in arcuate nucleus during long term malnutrition of female ovariectomized rats

Objective: Melanocortin-4 receptor (MC4R) and agouti-related peptide (AgRP) are involved in energy homeostasis in the rat. The aim of the present study was to evaluate the expression of MC4R and AgRP mRNAs in arcuate nucleus (ARC) during long term malnutrition of female ovariectomized rats. Materials and Methods: Ten female ovariectomized rats were divided into two equal groups (n=6) of normal ...

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Hypothalamic Expression of KiSS1 and RFamide-related Peptide-3 mRNAs during The Estrous Cycle of Rats

Kisspeptin and RFamide-related peptide-3 (RFRP-3) are known to affect GnRH/luteinizing hormone (LH) in several species, including the rat. It has been hypothesized that GnRH/LH changes during the rat estrous cycle may result from changes in the expression of KiSS1 and RFRP-3 genes. Therefore, the present study investigates KiSS1 and RFRP-3 gene expression at the transcriptional level in the rat...

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عنوان ژورنال:
international journal of molecular and cellular medicine

جلد ۳، شماره ۳، صفحات ۱۸۳-۱۸۹

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